Functional GUS assay of GRAS transcription factor from Medicago truncatula
نویسندگان
چکیده
منابع مشابه
High rooting frequency and functional analysis of GUS and GFP expression in transgenic Medicago truncatula A17
• An effective transformation method is described for Medicago truncatula A17, verifying its suitability as a model legume for functional genomics. • Media and culture methods are detailed that yielded an average frequency of 35% for recovery of transgenic shoots from cotyledonary node explants and 39% for root induction and regeneration of entire plants from 419 phosphinothricinresistant shoot...
متن کاملStrigolactone biosynthesis in Medicago truncatula and rice requires the symbiotic GRAS-type transcription factors NSP1 and NSP2.
Legume GRAS (GAI, RGA, SCR)-type transcription factors NODULATION SIGNALING PATHWAY1 (NSP1) and NSP2 are essential for rhizobium Nod factor-induced nodulation. Both proteins are considered to be Nod factor response factors regulating gene expression after symbiotic signaling. However, legume NSP1 and NSP2 can be functionally replaced by nonlegume orthologs, including rice (Oryza sativa) NSP1 an...
متن کاملMedicago truncatula ERN Transcription Factors: Regulatory Interplay with NSP1/NSP2 GRAS Factors and Expression Dynamics throughout Rhizobial Infection1[W]
Laboratory of Plant Microbe Interactions, Institut National de Recherche Agronomique (Unité Mixte de Recherche 441), Centre National de Recherche Scientifique (Unité Mixte de Recherche 2594), 31326 Castanet-Tolosan cedex, France (M.R.C., L.F., T.L., M.-C.A., A.N., D.G.B., J.F., F.d.C.-N.); and Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom (G.E....
متن کاملMedicago truncatula
Immanuel Kant noted this approach would not be suitable for understanding complex systems such as an organism. He said there would never be a " Newton of the blade of grass " : because the blade of grass is more than the sum of its parts, complete knowledge of the parts list will not necessarily be predictive of the behavior of the whole. Fortunately, we don't have to resort to a vitalistic exp...
متن کاملMedicago truncatula ERN transcription factors: regulatory interplay with NSP1/NSP2 GRAS factors and expression dynamics throughout rhizobial infection.
Rhizobial nodulation factors (NFs) activate a specific signaling pathway in Medicago truncatula root hairs that involves the complex interplay of Nodulation Signaling Pathway1 (NSP1)/NSP2 GRAS and Ethylene Response Factor Required for Nodulation1 (ERN1) transcription factors (TFs) to achieve full ENOD11 transcription. ERN1 acts as a direct transcriptional regulator of ENOD11 through the activat...
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ژورنال
عنوان ژورنال: Biotechnology & Biotechnological Equipment
سال: 2019
ISSN: 1310-2818,1314-3530
DOI: 10.1080/13102818.2019.1649096